Introduction

The ZytoFast® products are designed for outstandingly fast detection and discrimination of human pathogen viruses, e.g. HPV, EBV, CMV, and the determination of lymphocyte clonality by detecting Ig-κ and Ig-λ light chain RNA by Chromogenic in situ Hybridization (CISH) in formalin-fixed, paraffin-embedded tissue sections and cell samples. The signal intensity of ZytoFast® probes is increased even more when using the  ZytoFast® PLUS Implementation Kits.


Method Description - ZytoFast® zf method small

The ZytoFast® system uses oligonucleotide probes tagged with Biotin which are detected using enzyme-conjugated streptavidin targeting the tag. The enzymatic reaction of chromogenic substrates, e.g. BCIP/NBT or AEC, leads to the formation of strong color precipitates that can be visualized by light microscopy.

 

Method Description - ZytoFast® PLUS Zyto<em>Fast </em><sup>®</sup> PLUS Method Description

The ZytoFast® PLUS system uses Digoxigenin-labeled probes which are detected using primary antibodies. These antibodies are detected by polymerized enzyme-conjugated secondary antibodies. The enzymatic reaction of chromogenic substrates, e.g. NBT/BCIP, AEC or DAB, leads to the formation of strong color precipitates that can be visualized by light microscopy.

 

ZytoFast® - Flexibility that meets your needs 

 
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ZytoVision GmbH

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