Introduction

FlexISH® products are designed for identification of chromosomal aberrations on formalin-fixed, paraffin-embedded (FFPE) specimens by Fluorescence in situ Hybridization (FISH). Using the FlexISH® products gives you the flexibility to choose between a 1-day (2 h hybridization) or a 2-day (overnight hybridization) protocol by adapting the hybridization time just according to your individual needs! 

FlexISH®  brings Flexibility to your FISH

• FlexISH® maximizes your flexibility in terms of time and laboratory management.
• Hybridization time can be varied between 2 hours and overnight.
• With a hybridization temperature of 37°C the FlexISH® protocol is fully compatible with routine workflows in pathology laboratories.

Method Description & FlexISH®  Fluorochromes

The FlexISH® system uses direct-labeled FISH probes. The probes are detected by fluorescence microscopy using appropriate filter sets. Due to an exciter filter, full-spectrum light, emitted by the microscope lamp, is reduced to light of a defined wavelength that specifically excites the fluorophore of the probe. This light is reflected onto the specimen by a dichroic mirror. The fluorophore emits light of longer wavelengths that passes the mirror. Finally, a barrier filter reduces the emitted light to a defined wavelength that can be detected.

Two factors that mainly influence FISH analyses:

• Fluorochromes of the FISH probes
• Appropriate filter sets

Please refer to your local distributor for precise information regarding the suitable filter sets.

FlexISH®  Kits – Convenient Solutions

FlexISH® FISH probes are intended to be used with the:

• FlexISH®-Tissue Implementation Kit (Z-2182-5/-20) for FISH analyses on FFPE specimens